FastQCFastQC Report
Thu 2 Jun 2016
DRR028274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC4850.052.30933145
GGTATCA9400.047.8097721
TCAACGC12100.044.9292034
ATCAACG12400.043.842213
GTATCAA13750.042.1735461
CAACGCA13750.040.592045
AACGCAG15250.037.0746966
GTACATG26150.034.9262281
TATCAAC16750.034.187322
ACGCAGA16600.032.7496037
CGCAGAG17000.031.9790258
TACATGG27500.031.3665752
ACATGGG28650.029.0955163
TATAACG1100.009680008526.3584652
GAGTACA20050.026.0298081
GCAGAGT21950.023.7766579
CATGGGG21850.022.226764
GTATAGG3706.661139E-921.5498281
ATAACGC2053.670654E-421.2153513
TACCTGG9200.020.4851132
Produced by FastQC (version 0.11.5)