FastQCFastQC Report
Thu 2 Jun 2016
DRR028274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.12567002338431857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.11278382155093124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.063.8418051
GTATCAA20650.053.4593731
TCAACGC21100.050.8520854
ATCAACG21200.050.612223
CAACGCA22600.047.4769485
AACGCAG23750.045.4833226
TATCAAC26600.040.882662
ACGCAGA27950.038.3892337
GTACATG28550.037.9036061
CGCAGAG29350.036.558068
TACATGG28550.034.5352252
ACATGGG27900.033.2609983
TATAACG1102.436114E-432.95392
GCAGAGT35050.030.8196569
GTGGTAT7500.026.1458551
GTATATA7350.025.6913171
CATGGGG21250.025.5877344
GAGTACA20600.022.9164051
ATAACGC2003.1029005E-421.7495753
TATACAG8000.021.7495755
Produced by FastQC (version 0.11.5)