FastQCFastQC Report
Thu 2 Jun 2016
DRR028273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384431
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22250.045.2799031
TCAACGC21150.045.2360084
ATCAACG21250.045.0231323
CAACGCA24450.038.834095
GTACATG52750.038.0608141
AACGCAG26650.036.172226
TACATGG53650.035.6660422
GGTATCA15650.034.735031
TATCAAC29550.033.1128852
ACATGGG55450.032.9397053
GACGAAA4800.031.711332145
ACGCAGA31300.030.5668267
GCCGACA4250.028.99322145
CATGGGG40350.028.9203194
CGCAGAG32900.028.6396838
GAGTACA36550.025.5813271
GCAGAGT39050.023.201169
CCTATGC5350.021.676393
AGTACAT41650.020.8827172
ATGGGGG26050.020.3112135
Produced by FastQC (version 0.11.5)