FastQCFastQC Report
Thu 2 Jun 2016
DRR028273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384431
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.050.1181261
GTATCAA30700.044.4602431
TCAACGC30150.043.282234
ATCAACG31350.042.0883
CAACGCA32900.039.8847735
AACGCAG34450.038.0902526
TATCAAC38400.035.1160932
GTACCGT1751.0099575E-733.1418276
GTACATG50750.033.0467151
ACGCAGA40950.032.0441787
ACCGTCC2101.4411853E-831.0704638
CGCAGAG42350.030.813688
TACATGG54500.029.3981672
ACATGGG51450.029.1681883
GAGTACA38600.027.6491891
GTGGTAT13100.027.1567061
CTAACGC1100.00967431326.3628143
GCAGAGT51500.025.3390189
CATGGGG39300.024.9038054
GGTACCG1801.5210187E-424.1659155
Produced by FastQC (version 0.11.5)