FastQCFastQC Report
Thu 2 Jun 2016
DRR028272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066631
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.054.1211851
GTATCAA17450.046.9378931
TCAACGC17800.043.5716864
ATCAACG18450.041.6437723
TGACGCG1303.7051905E-739.0297145
CAACGCA22150.035.0147135
GTACATG37750.033.9857521
AACGCAG23550.033.5487336
TACATGG38350.032.5092
TATCAAC24650.031.7575782
ACGCAGA25200.030.7768237
ACATGGG40150.029.9683673
CGCAGAG26200.029.325488
GAGTACA29400.028.1059361
TCGCTCG1358.093668E-426.8458279
AGTACAT30250.025.1596412
TACTGCG2051.2926153E-524.7505447
GCAGAGT31750.024.6558829
TAGCACG1500.001496292424.1612434
CTTAGGG8850.022.932707145
Produced by FastQC (version 0.11.5)