FastQCFastQC Report
Thu 2 Jun 2016
DRR028272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066631
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.14381730889126604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.13041061060479212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.074.73371
GTATCAA32600.060.150231
ATCAACG36900.052.260323
TCAACGC37450.051.4928134
CAACGCA39550.048.9419865
TATCAAC41100.047.272632
AACGCAG41000.047.0342866
ACGCAGA46300.041.1804967
CGCAGAG47750.040.081818
GTGGTAT9300.039.8270721
TGGTATC9550.038.715392
GTACATG37500.037.1844251
GCAGAGT54850.035.157819
TACATGG37950.034.003612
ACATGGG38950.032.199973
GAGTACA28450.028.0802881
CATGGGG29400.025.6450064
AGTACAT29150.025.3675442
TAACCGT1500.00149476324.1654875
TACTTCG2552.7085134E-622.7439887
Produced by FastQC (version 0.11.5)