FastQCFastQC Report
Thu 2 Jun 2016
DRR028271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247746
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC3500.053.8397145
GGTATCA11750.044.4111441
ATCAACG16400.043.309143
TCAACGC16500.042.6074074
GTATCAA18150.039.9319651
GTACATG40600.037.130831
CAACGCA20200.035.5206685
TACATGG43600.034.077262
AACGCAG21700.033.399326
ACATGGG44850.032.804313
ACGCAGA22700.031.60877
TATCAAC23200.030.9274792
GAGTACA31550.030.782421
TCTCGGG9250.029.774136145
CGCAGAG23800.029.538758
CATGGGG33200.027.2878484
TCTAGCG1100.0096877726.3550973
AGTACAT34250.026.2396742
GCAGAGT28700.023.7379539
TATACGG1902.198409E-422.8873212
Produced by FastQC (version 0.11.5)