FastQCFastQC Report
Thu 2 Jun 2016
DRR028271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247746
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.055.7174451
ATCAACG24800.049.695943
TCAACGC25000.049.2983674
GTATCAA26500.047.126811
CAACGCA26850.045.901645
AACGCAG29400.042.166976
GTACATG40350.039.22821
TATCAAC32200.038.9505732
ACGCAGA33150.037.396957
CGCAGAG33500.037.2226458
TACATGG41650.036.2052842
ACATGGG41950.034.5638123
GTCGTAC1652.3594603E-630.8034631
GCAGAGT39900.030.7070039
CATGGGG29850.029.3876364
TATAACG1306.4851134E-427.8836922
GAGTACA29200.024.6171511
GTGGTAT11050.023.655151
CGTACAC1600.002175869222.6554993
GGTACCG1952.6158334E-422.3069536
Produced by FastQC (version 0.11.5)