FastQCFastQC Report
Thu 2 Jun 2016
DRR028270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460034
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.048.5938451
TCAACGC21800.046.8785864
ATCAACG21750.046.6531223
GTATCAA24500.044.374871
GTACATG52100.039.786911
CAACGCA27200.037.038885
TATCAAC28350.036.559052
ACATGGG53250.036.0693363
AACGCAG27950.036.044996
TACATGG55150.035.6152232
ACGCAGA29800.033.564087
CATGGGG44600.030.5516664
CGCAGAG32450.030.376398
GAGTACA36400.029.4694631
GCAGAGT39800.024.2203549
AGTACAT42000.024.1596532
GCTAGAC3402.5156623E-923.4490743
ATGGGGG29350.022.7191285
TCCGATA2755.2264986E-621.0847872
AGAGTAC31750.019.8603745
Produced by FastQC (version 0.11.5)