FastQCFastQC Report
Thu 2 Jun 2016
DRR028270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460034
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.058.4410861
GTATCAA31950.049.0980151
ATCAACG31650.049.018723
TCAACGC31900.048.634564
CAACGCA34450.045.0346155
AACGCAG35850.043.275946
TATCAAC39050.040.4722482
ACGCAGA41300.037.2141157
GTACATG53800.037.1220131
CGCAGAG43750.034.798718
TACATGG56800.034.7170222
ACATGGG54950.033.511043
GAGTACA37250.031.1942671
GCAGAGT52000.029.2777619
TAGACCG1050.007710987727.6180255
CATGGGG45950.027.4527364
AGTACAT42350.026.7050322
CTAGCGT1100.00967476126.362664
GTGGTAT13250.025.2129151
GTATACG1600.00215409722.695051
Produced by FastQC (version 0.11.5)