FastQCFastQC Report
Thu 2 Jun 2016
DRR028269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123614
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC4700.055.526443145
TCAACGC16600.048.9086654
GTATCAA17450.048.187951
GGTATCA12700.047.9458961
ATCAACG17500.046.3933643
CGACGAA3700.043.103863145
GTACATG40100.039.227561
CAACGCA21000.039.0063255
TACATGG40150.037.5537832
TATCAAC23500.035.782122
AACGCAG22600.035.6033136
ACATGGG42350.034.0624243
ACGCAGA24800.032.737257
CGCAGAG24450.032.6129238
GTACGGA1204.061765E-430.2040126
GAGTACA26850.028.3479021
CATGGGG31700.026.7548484
GTACACG2001.0668045E-525.371371
GCAGAGT31150.024.900139
TCTCGGG11000.023.06591145
Produced by FastQC (version 0.11.5)