FastQCFastQC Report
Thu 2 Jun 2016
DRR028269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123614
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.11827905312678554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.1073322333114397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.071.307641
GTATCAA30450.059.151991
ATCAACG34800.050.4136353
TCAACGC35150.050.1178974
CAACGCA36950.047.6764265
AACGCAG39550.045.2754066
TATCAAC39500.044.5985832
ACGCAGA44750.039.528357
CGCAGAG45150.039.017598
TACATGG40400.035.530042
GTACATG40600.035.0618631
ACATGGG41000.033.5955433
GCAGAGT52850.032.9213879
GTGGTAT11850.030.6447891
CATGGGG32400.027.2977394
TGGTATC13250.026.2625712
TATACGG1701.0318253E-425.5866975
GAGTACA29250.024.5818351
TAGGGTG7250.022.9986155
CCGCGTG1902.1942964E-422.893369
Produced by FastQC (version 0.11.5)