FastQCFastQC Report
Thu 2 Jun 2016
DRR028268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906535
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.052.599891
GTATCAA16100.045.9223141
TCAACGC15750.043.261024
ATCAACG16250.042.8220373
CAACGCA17200.040.035445
AACGCAG19100.036.0528566
GTACATG34500.034.6668431
TATCAAC21350.032.5928842
ACGCAGA21350.032.5928847
TACATGG36200.031.436962
CGCAGAG22100.031.1588048
ACATGGG35800.030.978323
GTCTACG1204.0625993E-430.2021751
GAGTACA26250.029.2702221
TACCTGG10600.028.0367342
GCAGAGT26000.025.9274049
CATGGGG26150.024.9471114
AGTACAT29150.024.3689582
TACCGTC1500.001495940824.161747
GTCCTAG4303.6379788E-1223.5998381
Produced by FastQC (version 0.11.5)