FastQCFastQC Report
Thu 2 Jun 2016
DRR028268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906535
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.12354735338403922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.11946587831688793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.063.1867331
GTATCAA25450.053.0909421
TCAACGC28350.046.5425724
ATCAACG29050.045.670633
AACGCAG29700.045.403426
CAACGCA29400.045.126935
TATCAAC33200.040.398542
ACGCAGA34950.037.9608547
CGCAGAG36550.036.299098
GTACATG36000.035.1109051
TACATGG37200.033.1312682
GTGGTAT9100.032.7293781
GCAGAGT41900.031.6642469
ACATGGG37400.031.597163
TACCTGG8250.028.9996032
CATGGGG27050.028.677984
GTATAGG4550.025.544881
GAGTACA26700.024.2144171
CAGAGTA40550.023.778969
GTTATAG4205.0931703E-1122.4848181
Produced by FastQC (version 0.11.5)