FastQCFastQC Report
Thu 2 Jun 2016
DRR028267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269790
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20000.051.826451
TCAACGC19300.051.4527634
ATCAACG20450.048.559333
CAACGCA23500.042.2569475
AACGCAG24850.040.2529876
GGTATCA13050.039.9914741
GTACATG45900.039.321681
TATCAAC26300.038.3093222
ACGCAGA26450.037.2699367
TACATGG47500.036.0133742
CGCAGAG27850.034.8758668
ACATGGG50400.032.790633
TAGATCG2101.44355E-831.0648065
GAGCGAC5400.030.873047145
CATGGGG40150.027.6217584
GAGTACA34250.027.0891131
GCAGAGT34900.026.9999719
AGTACAT34950.025.9243722
GTATTGG8950.019.4371971
CTAGTAC3051.277734E-519.012343
Produced by FastQC (version 0.11.5)