FastQCFastQC Report
Thu 2 Jun 2016
DRR028267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269790
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.058.2715871
GTATCAA27800.054.604161
TCAACGC29600.050.6988834
ATCAACG30200.049.4515573
CAACGCA31450.047.4860765
AACGCAG32800.045.5316166
TATCAAC36500.041.1147082
ACGCAGA38050.039.2493327
CGCAGAG39850.037.4764678
GTACATG46950.035.1167641
TACATGG47650.032.8632552
GCAGAGT47950.031.2969079
GTATAGG6300.031.127731
ACATGGG48750.030.3371853
GAGTACA36000.026.8332581
CATGGGG37100.025.7940644
GTGGTAT9400.025.4982471
AGTACAT36850.024.591912
GTACGGA2202.198426E-523.1099821
TATAGGG6500.022.3067532
Produced by FastQC (version 0.11.5)