FastQCFastQC Report
Thu 2 Jun 2016
DRR028266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154497
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.052.030371
TCAACGC17750.051.0458154
ATCAACG18300.049.9077453
GGTATCA12000.047.115291
CAACGCA22300.040.6306345
TATCAAC23050.040.2520072
GACGACC6500.040.14557145
AACGCAG24050.036.7699666
GTACATG38200.035.1040231
ACGCAGA25800.032.871137
TACATGG40050.032.758542
CGCAGAG26050.032.555678
ACATGGG42500.029.5056843
GAGTACA32050.028.0441421
GCAGAGT30350.026.7490169
AGTACAT31650.026.5664042
TGTACCG1751.2576488E-424.8520185
CATGGGG32150.024.575034
TACACTG12800.023.217875
AGTCCGC2502.283814E-623.195219145
Produced by FastQC (version 0.11.5)