FastQCFastQC Report
Thu 2 Jun 2016
DRR028266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154497
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.066.500891
GTATCAA27700.060.303161
TCAACGC30800.053.6666154
ATCAACG31250.052.6618273
CAACGCA32500.050.859445
AACGCAG35600.046.430676
TATCAAC36900.044.9913672
ACGCAGA40000.040.05467
CGCAGAG41600.038.688318
GTACATG40650.038.23361
TACATGG42550.034.7576752
GTGGTAT10400.033.519681
GCAGAGT47500.033.424949
ACATGGG45000.031.5764733
TAGATCG1255.15557E-428.9988065
TGGTATC11900.025.587182
GTATACG2051.2732402E-524.7991141
GAGTACA32200.024.584571
CATGGGG34800.024.582324
TATACGG2101.5558358E-524.1656682
Produced by FastQC (version 0.11.5)