FastQCFastQC Report
Thu 2 Jun 2016
DRR028265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060293
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG14300.046.634853
TCAACGC14850.044.4195024
GTATCAA16650.043.1002431
GGTATCA12200.040.9966161
GTACATG35600.038.0759161
CAACGCA17500.037.6931235
TACATGG36900.034.770082
AACGCAG19350.034.4639976
ACATGGG37400.033.3361633
TATCAAC21550.032.6274572
ACGCAGA21250.031.0413957
CGCAGAG21250.030.700288
GACGAAA4300.030.343298145
CATGGGG27050.030.0130084
GCCGACA3306.184564E-1126.358826145
GAGTACA24400.024.9544621
GCAGAGT26500.023.7975439
GGTACCG1952.6181183E-422.3036236
TCGTGCG1700.003096191221.319649
AGTACAT28000.021.2282682
Produced by FastQC (version 0.11.5)