FastQCFastQC Report
Thu 2 Jun 2016
DRR028265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060293
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.060.1204951
GTATCAA26900.054.779761
ATCAACG28350.050.3774533
TCAACGC28350.050.121734
CAACGCA30100.047.2076725
AACGCAG32250.044.0604976
TATCAAC33550.042.5693242
ACGCAGA37150.038.0538677
GTACATG40050.037.155891
CGCAGAG37900.037.109548
TACATGG40900.034.210312
GTGGTAT9850.033.8998641
ACATGGG41200.032.905423
GCAGAGT43300.031.6444079
CATGGGG31300.027.3313264
TGGTATC12950.025.752012
GAGTACA28850.025.6643771
ATAATAC7650.023.6919943
AGTACAT31950.022.2371042
GTATAGG5950.021.9599781
Produced by FastQC (version 0.11.5)