FastQCFastQC Report
Thu 2 Jun 2016
DRR028264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182483
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.052.2897381
ATCAACG20800.048.785173
GTATCAA21600.048.656111
TCAACGC21150.047.6351554
CAACGCA25400.039.950065
GTACATG45900.039.1617551
TATCAAC26450.038.364142
AACGCAG27200.037.3063056
TACATGG48400.035.64142
ACGCAGA28150.035.0173767
CGCAGAG29000.033.4911428
ACATGGG52100.032.5537643
GAGTACA33750.026.844751
TGACGCG1701.0333044E-425.581467145
GCAGAGT36650.025.3139029
CATGGGG39700.025.1948474
AGTACAT34800.022.7023262
TATTACG1952.6198194E-422.3017922
GTGGTAT8500.021.3178881
CAGAGTA33300.018.9364324
Produced by FastQC (version 0.11.5)