FastQCFastQC Report
Thu 2 Jun 2016
DRR028264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182483
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.070.456091
GTATCAA29300.060.247551
ATCAACG32750.053.3487323
TCAACGC33150.052.4863174
CAACGCA35950.048.398375
AACGCAG36600.047.7369166
TATCAAC38500.045.5693662
ACGCAGA42900.040.557617
CGCAGAG45000.038.503818
TAGGTCG1051.8544124E-434.522255
GTACATG44350.034.2336651
GCAGAGT53350.031.7980029
TACATGG47100.030.784172
ACATGGG48050.029.873783
CATGGGG36000.026.9848924
GAGTACA34150.025.313781
ATATCGC1450.001226898924.998876
AGTACAT34250.024.1302972
GTGGTAT9450.021.5241991
CAGAGTA48750.020.819579
Produced by FastQC (version 0.11.5)