FastQCFastQC Report
Thu 2 Jun 2016
DRR028263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611447
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC2100.051.76933145
TATAACG803.7399222E-545.2981642
GGTATCA5650.044.8972931
TCAACGC7500.042.5198754
ATCAACG7900.041.28443
GTATCAA8600.039.609561
CAACGCA8350.038.1915055
AGTCCGC1001.3934082E-436.23853145
GTACATG18050.036.1381451
TACCTGG6450.035.957612
TCTCGGG4800.033.21865145
AACGCAG9700.032.8761946
TACATGG18850.031.9129752
TATCAAC11350.029.3739642
ACATGGG19850.028.479653
CTGTGCG1050.00771805527.610319
ACCTGGG8150.027.5679633
CGCAGAG11900.027.4072938
GTACCTG8200.027.3998641
ACGCAGA11650.027.3733127
Produced by FastQC (version 0.11.5)