FastQCFastQC Report
Thu 2 Jun 2016
DRR028263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611447
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8200.061.0845071
GTATCAA12800.052.743491
TCAACGC14150.047.136644
ATCAACG14650.045.0330123
CAACGCA15000.044.465565
AACGCAG15800.042.6729936
TATCAAC16450.040.105392
TACCTGG7950.038.300942
ACGCAGA17750.037.9849747
CGCAGAG18150.037.147848
GTACGCC1051.8531462E-434.5229533
GTGTACG850.002723945534.16151
GTACATG18500.032.961231
GTACCTG9500.032.093831
TACATGG18350.031.6068442
TATAACG1402.7454109E-531.0706562
ACCTGGG9900.030.0245083
GCAGAGT23150.029.124559
TATACGG1000.006073373428.9992795
ACATGGG19250.028.2460543
Produced by FastQC (version 0.11.5)