FastQCFastQC Report
Thu 2 Jun 2016
DRR028262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909738
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15100.054.2444154
GTATCAA16100.051.325421
ATCAACG16400.049.9445573
GGTATCA10300.048.5585171
CAACGCA17100.047.9000365
AACGCAG18400.044.5157976
TATCAAC20800.039.379362
ACGCAGA20450.038.635527
CGCAGAG21500.036.7486658
GTACATG33000.036.242951
TACATGG33050.034.6528662
ACATGGG34800.031.2439213
GCAGAGT25950.029.3295569
CATGGGG25200.027.3260354
GAGTACA23700.025.9970531
GTCTTAG5100.022.7406751
GTATAGG4800.022.6518441
AGTACAT26800.021.6375832
AACACCG2104.3193842E-420.7102585
TATACAG8350.019.9661755
Produced by FastQC (version 0.11.5)