FastQCFastQC Report
Thu 2 Jun 2016
DRR028262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909738
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.12992751759297733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.11146066230057444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.063.228251
GTATCAA24300.044.5288351
TCAACGC26550.040.958824
ATCAACG26700.040.4571953
CAACGCA27900.038.976945
AACGCAG29800.036.2485586
TATCAAC30500.035.1789252
GTACATG32050.033.308161
TACATGG32500.032.3448642
CTACGCT900.00362876732.220944
GTGGTAT9450.029.9705091
ACATGGG34650.029.7102183
ACGCAGA35750.029.6072147
CGCAGAG35400.029.490358
CATGGGG24300.027.745814
GAGTACA25250.027.035091
CGCTTAA1409.897228E-425.9360181
GCAGAGT40700.025.2938339
CTCCGAC3001.704393E-824.169691145
AGTACAT26750.024.1205372
Produced by FastQC (version 0.11.5)