FastQCFastQC Report
Thu 2 Jun 2016
DRR028261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187465
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC5200.059.939335145
GGTATCA12000.042.8868261
TCAACGC14900.042.8098034
ATCAACG15700.040.628413
GTATCAA17500.039.763081
GTACATG36750.037.080651
TACATGG37550.034.93942
CAACGCA18350.034.7610935
AACGCAG19950.032.3365676
ACATGGG39850.031.467673
ACGCAGA21900.029.4572857
CGCAGAG22000.029.3233878
TATCAAC22400.029.1233462
GAGTACA27300.026.2856881
CATGGGG30850.026.0804214
GTATACG1400.001000795425.8874191
CGACGAA3108.60382E-1025.720406145
TATATCG1751.2577113E-424.8519255
ATACCGT2552.7127371E-622.7403226
GCAGAGT29650.021.5131879
Produced by FastQC (version 0.11.5)