FastQCFastQC Report
Thu 2 Jun 2016
DRR028261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187465
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.053.750511
GTATCAA28000.051.619051
ATCAACG28700.048.5006183
TCAACGC28800.048.332214
CAACGCA31350.044.400885
AACGCAG33500.041.7676856
TATCAAC34700.040.3232732
ACGCAGA37950.036.4879537
CGCAGAG37650.036.2010158
GTACATG39250.035.7135161
TACATGG39550.033.5453642
ACATGGG40850.032.300353
GTATAGG6550.028.8301561
GCAGAGT47600.028.1768659
GTGGTAT12650.024.6883981
CATGGGG30400.023.848134
TGGTATC12950.022.9531352
GAGTACA30050.022.2360841
CAGAGTA44100.021.0425959
TACACTG12550.019.6409785
Produced by FastQC (version 0.11.5)