FastQCFastQC Report
Thu 2 Jun 2016
DRR028260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293340
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14750.053.068314
ATCAACG15550.050.338113
GTATCAA18100.046.4497031
CAACGCA18600.042.863075
AACGCAG20550.038.7957736
GTACATG37450.038.5127751
ACGCAGA21650.036.1550877
GGTATCA12700.035.9534341
CGCAGAG22100.034.762998
TATCAAC22800.034.649362
TACATGG39950.034.1070862
ACATGGG41100.031.9183393
TAGATCG950.00473496830.5168675
GTATACG1358.0986327E-426.8435381
CATGGGG29550.026.2439884
GAGTACA28850.025.875871
GCAGAGT30450.024.0401749
TCTCGGG10350.022.40852145
GGATAGG9400.021.58906145
AGTACAT30700.021.4835762
Produced by FastQC (version 0.11.5)