FastQCFastQC Report
Thu 2 Jun 2016
DRR028260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293340
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.062.180811
TCAACGC26250.053.5791664
GTATCAA27350.053.1029621
ATCAACG26600.052.874183
CAACGCA28300.049.9541665
AACGCAG30500.046.350916
TATCAAC34700.041.3674962
ACGCAGA35100.039.4502537
CGCAGAG35650.038.6382648
GTACATG38550.034.849251
TACATGG38300.032.7469522
GCAGAGT43350.031.1062289
ACATGGG38050.030.6757183
GAGTACA29500.030.5243051
GCCGTGC2705.5679266E-926.8509568
AGTACAT31550.026.6552142
GTACCGG1500.001480384724.2061021
CATGGGG29350.023.4664
GTCTAAC2802.3071334E-723.3415991
GTATAGG7450.022.4190731
Produced by FastQC (version 0.11.5)