FastQCFastQC Report
Thu 2 Jun 2016
DRR028259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906420
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.050.5798571
TCAACGC13400.047.058184
ATCAACG13850.046.052543
GGTATCA9100.045.3998221
CAACGCA15750.040.03685
GTACGAC750.001485283438.6562233
AACGCAG17500.037.2756426
TATCAAC18250.036.140922
ACGCAGA18700.033.333247
GTACATG30200.031.9201831
CGCAGAG19650.031.3528548
TACATGG32000.028.7656652
GAGCGAC3403.6379788E-1227.7131145
GAGTACA22800.027.6570011
ACATGGG32700.027.2632773
TATAACG1450.001228287624.9932482
AGTACAT24950.024.1117212
ATGGGCG2151.8693905E-523.5982765
GCAGAGT25200.023.5848989
TGTACGA1851.8338369E-423.5071622
Produced by FastQC (version 0.11.5)