FastQCFastQC Report
Thu 2 Jun 2016
DRR028259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906420
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.11164802188830786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.11131704949140575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.068.1391141
GTATCAA22400.048.959661
TCAACGC24000.044.7062954
ATCAACG24400.043.97343
CAACGCA25700.041.749075
GTACATG29000.039.3197861
AACGCAG28100.038.4413076
TATCAAC29400.037.2346952
TACATGG29950.037.0350342
ACATGGG30150.035.106193
ACGCAGA32200.032.871177
CGCAGAG33150.032.1478548
TATAACG1703.0123774E-629.8515782
GCAGAGT38900.026.836829
GTATAGG4650.026.5525041
ACCGGAC1100.00967326726.3624343
ATACCGC1100.00967326726.3624346
CAGAGTA37800.025.5080959
CATGGGG24650.025.2929634
CTCCGAC3451.07320375E-1025.223198145
Produced by FastQC (version 0.11.5)