FastQCFastQC Report
Thu 2 Jun 2016
DRR028258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228800
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.047.2467271
TCAACGC18250.046.0699734
GTATCAA19950.045.414021
ATCAACG18600.045.203063
GACGACC7050.043.180035145
CAACGCA22000.038.8760575
GTACATG45650.035.724371
AACGCAG24150.035.7151646
TATCAAC26450.032.8835262
TACATGG47150.032.7431682
ACGCAGA25950.032.3998837
CGCAGAG26950.030.9287178
ACATGGG49400.030.518223
TAGATCG1453.497364E-529.9920445
GAGTACA33900.029.0778351
CATGGGG38400.026.0477774
AGTACAT36450.025.2539332
TCGTGCG1450.00122850124.993379
GCAGAGT34250.024.5482339
GTATAAG7450.024.3224091
Produced by FastQC (version 0.11.5)