FastQCFastQC Report
Thu 2 Jun 2016
DRR028258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228800
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.065.146861
GTATCAA27750.054.9392551
ATCAACG29950.049.138793
TCAACGC29950.049.138794
CAACGCA31800.046.2800835
AACGCAG34500.042.86836
TATCAAC37050.040.1135252
ACGCAGA40350.036.8328677
CGCAGAG42000.034.6954048
GTACATG43150.034.4905051
TACATGG44700.030.4912722
GCAGAGT47850.029.9991139
ACATGGG46550.028.8122583
TAGATCG1358.0851617E-426.8510595
GAGTACA35000.026.7576621
TATACTG9750.023.794175
CATGGGG36800.023.4436044
AGTACAT36800.023.2465972
GTGGTAT10050.023.1158771
CCTATAC4403.6379788E-1223.0675033
Produced by FastQC (version 0.11.5)