FastQCFastQC Report
Thu 2 Jun 2016
DRR028254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015799
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.053.7115171
GTATCAA16000.044.3928951
ATCAACG14900.044.265213
TCAACGC15300.043.1079444
GTACATG38450.039.207971
TACATGG38800.037.1730962
CAACGCA17800.037.053465
GAGTACA26700.035.5604631
ACATGGG40000.034.2459453
AACGCAG19800.034.042796
TATCAAC20050.033.6183132
AGTACAT29050.031.4363272
ACGCAGA21400.030.4814827
CGCAGAG21450.029.7346428
CATGGGG30200.028.07934
GTCGTCC1701.0334188E-425.580543
ATAATAC8200.023.8647733
CTAGTAC3052.0412699E-823.7633443
GTACTAG3152.874367E-823.008951
GCAGAGT27550.022.887859
Produced by FastQC (version 0.11.5)