FastQCFastQC Report
Thu 2 Jun 2016
DRR028254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015799
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.066.153731
GTATCAA23600.049.8492471
TCAACGC24050.047.3274044
ATCAACG24450.046.5531353
CAACGCA25750.044.2028775
AACGCAG27200.042.1130146
TATCAAC31100.036.5988432
GTACATG34350.036.1513861
ACGCAGA32100.035.45877
TACATGG35000.034.3849332
ACATGGG36050.032.579013
CGCAGAG34300.032.3389178
GAGTACA27450.029.6299721
GCAGAGT39500.026.98049
GAGTACG2756.6465873E-926.4072321
AGTACAT29250.026.025052
CATGGGG27650.025.1712174
GTATAAT8650.023.5070151
CCTAGTA4403.6379788E-1223.0676572
ATAATAC9050.022.4304283
Produced by FastQC (version 0.11.5)