FastQCFastQC Report
Thu 2 Jun 2016
DRR028252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225893
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.050.544881
ATCAACG18000.046.3096583
TCAACGC18600.044.81584
GTATCAA21900.041.0415571
GTACATG40100.039.9479181
CAACGCA22000.037.889725
TACATGG40350.037.005812
AACGCAG23400.035.932586
TATCAAC23800.035.633232
ACATGGG42900.032.102773
ACGCAGA26400.031.3002057
CGCAGAG26750.030.0777558
TCTCGGG11350.026.823622145
CATGGGG31050.026.3792924
GAGTACA28250.025.6582951
GCAGAGT30600.025.1090749
TAAATCG1450.001228113224.9947195
CCGTGCA6250.023.1950999
AGAGTAC26700.022.2612135
AGTACAT31400.021.4683932
Produced by FastQC (version 0.11.5)