FastQCFastQC Report
Thu 2 Jun 2016
DRR028252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225893
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.060.825741
GTATCAA31400.047.4256481
ATCAACG30250.047.212943
TCAACGC30500.047.063644
CAACGCA32900.043.630435
AACGCAG36250.039.7983636
TATCAAC36650.039.5618132
ACGCAGA40000.035.8860247
CGCAGAG40200.035.527158
GTACATG41300.032.8912661
GGACCGT2008.958523E-932.623666
GTGGTAT11700.031.6645531
TACATGG42250.030.8863052
GCAGAGT46200.030.7563139
ACATGGG42250.029.6851713
TCTAGCG1000.00607620528.998812
GTACGGA1504.4007415E-528.9988086
GAGTACA34400.028.2966731
GTACTAG4050.026.904521
AGTACAT35950.024.6026062
Produced by FastQC (version 0.11.5)