FastQCFastQC Report
Thu 2 Jun 2016
DRR028251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335417
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.050.5840231
TCAACGC21050.042.7011874
ATCAACG21800.041.232113
GTATCAA23350.040.04731
GTACATG45550.038.512111
CAACGCA24250.037.066395
TACATGG46900.036.0125162
ATTACGC1051.8557455E-434.5184363
AACGCAG25850.034.4917266
ACATGGG48000.034.4321373
GAGCGAC3600.034.23078145
ACGCAGA28300.030.9934087
TATCAAC28800.030.958722
CGCAGAG29350.029.6376328
CATGGGG34800.027.9123214
GCAGAGT36300.022.9647459
GAGTACA34950.021.7777231
AGAGTAC31950.020.4193555
TTACGAT2155.062669E-420.2294084
GTATAGG6900.019.9606591
Produced by FastQC (version 0.11.5)