FastQCFastQC Report
Thu 2 Jun 2016
DRR028251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335417
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32700.055.5425031
GGTATCA20150.055.5237581
TCAACGC34150.052.6480874
ATCAACG34100.052.5126843
CAACGCA35850.050.1515275
AACGCAG38100.047.1898236
TATCAAC40050.045.4352382
ACGCAGA43600.041.403267
CGCAGAG45050.039.748788
GTACATG44100.039.5371931
TACATGG44600.037.3864822
GCAGAGT51400.034.556099
ACATGGG46500.033.0525173
GTGGTAT12650.029.8638651
TACCGTA1952.6092675E-729.742477
CATGGGG34150.026.3240434
GTATACG2151.8368108E-523.6533571
GTATAGG6500.023.4714091
GAGTACA32900.023.406861
TAGATCG1550.001809202523.3862135
Produced by FastQC (version 0.11.5)