FastQCFastQC Report
Thu 2 Jun 2016
DRR028250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333699
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19500.047.5758633
TCAACGC19650.046.843844
GGTATCA14400.046.8092581
GTATCAA21650.044.5251161
GACGACC8100.043.845234145
GTACATG47900.039.946591
TATCAAC26400.036.513972
TACATGG50250.036.4918442
CAACGCA25600.035.9563065
ACATGGG51200.035.1069453
ACGCAGA26500.035.0086567
CGCAGAG27700.033.2303778
AACGCAG27850.033.05146
CATGGGG39400.029.8009514
GAGTACA34150.026.5295961
GCAGAGT37000.024.681999
GTATAGG5550.022.2007311
AGTACAT38650.022.1280862
AGAGTAC30850.021.614445
ATGGGGG26550.021.2932225
Produced by FastQC (version 0.11.5)