FastQCFastQC Report
Thu 2 Jun 2016
DRR028250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333699
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.067.352431
GTATCAA30550.052.5472341
ATCAACG32450.048.2573
TCAACGC32800.047.7420654
CAACGCA34750.045.0630155
AACGCAG37950.041.4542666
TATCAAC38550.040.8090632
GTACATG51400.040.559031
ACGCAGA40800.038.5585637
TACATGG53450.037.571052
CGCAGAG42400.036.7615558
ACATGGG55000.035.3259123
GTCTAGC3150.032.2839161
GCAGAGT52300.029.525639
GTCGTAC1255.0973543E-429.0555291
CATGGGG42000.028.9988844
TAGATCG1606.831607E-527.1864535
GAGTACA36750.027.0789621
TCGTACA1751.2563614E-424.8561862
AGTACAT39450.024.4413912
Produced by FastQC (version 0.11.5)