FastQCFastQC Report
Thu 2 Jun 2016
DRR028249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272784
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17750.045.324714
ATCAACG18800.042.793283
GTATCAA20450.042.1758731
GTACATG41900.040.304391
CAACGCA20650.038.95955
TACATGG43000.037.756352
GGTATCA13950.037.4083631
AACGCAG22300.036.4018636
ACATGGG44450.035.5463643
TAGTGCG850.002746217234.1076247
TATCAAC25300.033.2313422
ACGCAGA25050.031.8269777
CGCAGAG26050.030.3269848
CATGGGG33750.028.1324774
GCAGAGT33000.022.8417749
TCTCGGG14000.022.779919145
GAGTACA30150.021.6354351
CATGGGA17200.021.0693914
TATACTG9600.020.3846365
CAGAGTA32200.019.8078449
Produced by FastQC (version 0.11.5)