FastQCFastQC Report
Thu 2 Jun 2016
DRR028249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272784
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.061.3022961
GTATCAA28400.054.2066651
TCAACGC28700.051.2781264
ATCAACG28900.050.9232643
CAACGCA30050.049.2157025
AACGCAG33300.044.8477826
TATCAAC34550.043.2252162
CGCAGAG38450.037.5210278
ACGCAGA38650.037.5144467
GTACATG42850.034.9101371
TACATGG44950.031.9340132
ACATGGG45550.030.8767363
GCAGAGT46700.030.5821029
CATGGGG33400.026.6978724
CTCCGAC4400.026.371748145
GTATAAC5300.024.6621991
GAGTACA33600.023.3410111
TACACCG3503.5051926E-922.7846665
GTGTAAG9250.021.9812031
CCTATAC6000.021.7489993
Produced by FastQC (version 0.11.5)