FastQCFastQC Report
Thu 2 Jun 2016
DRR028248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG17700.056.922643
GTATCAA18150.056.3100621
TCAACGC18100.056.065154
GGTATCA10150.045.704341
TATCAAC22600.044.581012
CAACGCA22800.044.1899455
AACGCAG23850.042.2444766
TGACGCG2100.041.419563145
ACGCAGA24850.039.9611247
CGCAGAG25150.039.772668
GTACATG38150.036.0996131
GTATACG1458.7137596E-734.9923861
TACATGG38300.034.4442022
TACCGTC1153.166236E-431.5148817
GCAGAGT31350.030.9820949
ACATGGG42200.030.4021533
CATGGGG33750.025.772174
GAGTACA30750.024.7507131
GTATTAG7500.023.1949541
GATACCG1600.002177969322.6513215
Produced by FastQC (version 0.11.5)