FastQCFastQC Report
Thu 2 Jun 2016
DRR028248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.1299905374535236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.11957217820173385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.078.253431
GTATCAA29200.059.198771
TCAACGC32650.051.068494
ATCAACG33500.049.772723
CAACGCA34450.048.400185
AACGCAG36650.045.692656
TATCAAC37400.044.7763562
ACGCAGA42250.039.293187
CGCAGAG43450.038.0411348
GTACATG38800.035.004871
GCAGAGT49350.032.9055569
TACATGG40000.032.804012
GTGGTAT8200.031.0007921
ACATGGG43100.029.6035483
GAGTACA29800.028.0285221
TACGCGC1100.00967500126.361846
AGTACAT31600.025.9238642
ACGCGCT1500.001494913124.1650187
CAGAGTA46150.023.7199429
TGGTATC9500.022.8931752
Produced by FastQC (version 0.11.5)