FastQCFastQC Report
Thu 2 Jun 2016
DRR028247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434256
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC4050.057.27252145
GGTATCA13500.056.3776361
TCAACGC20600.047.1507844
GTATCAA22100.046.90241
ATCAACG21350.045.4944343
CAACGCA24900.039.2993855
AACGCAG26300.037.4830136
GTACATG44350.034.6492271
TATCAAC28900.034.612472
ACGCAGA27650.034.6043057
CGCAGAG29350.032.106038
TACATGG46700.031.1982692
ACATGGG49900.028.1807483
GCAGAGT37400.024.614079
GAGTACA37150.022.438321
TCTCGGG11350.021.713728145
GTATATA10400.021.6062641
CCTATAC6750.021.4771963
AGTACAT37800.021.2854352
CATGGGG35300.020.7394874
Produced by FastQC (version 0.11.5)