FastQCFastQC Report
Thu 2 Jun 2016
DRR028247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434256
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.054.600311
TCAACGC25500.046.341894
GTATCAA26200.046.030161
ATCAACG25800.045.2410283
CAACGCA27700.042.399585
AACGCAG30750.038.901396
TATCAAC32700.035.694762
GTACATG45650.035.6486321
ACGCAGA34150.034.6037527
CGCAGAG35250.033.523928
TACATGG47700.032.525332
ACATGGG49650.029.6416993
GCAGAGT43950.026.3929189
GAGTACA34100.024.5007361
GTGGTAT11000.024.4368211
TTTCGCG1851.831541E-423.512889
CTCCGAC3905.020411E-1022.309425145
AGTACAT37100.021.1045532
GTATAAT10800.020.8532431
CATGGGG36750.020.7137264
Produced by FastQC (version 0.11.5)