FastQCFastQC Report
Thu 2 Jun 2016
DRR028246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200715
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20150.050.7215041
GGTATCA13600.049.0339621
ATCAACG20500.048.0876053
TCAACGC20650.047.7382974
CAACGCA25050.039.0637665
AACGCAG26400.037.0661856
TATCAAC27650.036.4391062
GTACATG44500.036.323941
ACGCAGA27300.035.5787127
TACATGG45150.034.356122
CGCAGAG28550.034.0209738
ACATGGG49100.030.8540973
GAGTACA33400.029.2978251
GCAGAGT35200.026.9759489
AGTACAT36150.025.2644792
CATGGGG36700.024.4908434
GTGGTAT10500.021.400331
GTATTAG9300.020.264621
TGCACCG3351.3174722E-619.473585
CATGGGA16600.018.3395754
Produced by FastQC (version 0.11.5)