FastQCFastQC Report
Thu 2 Jun 2016
DRR028246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.067.627041
GTATCAA27900.050.256621
TCAACGC31950.042.885234
ATCAACG31800.042.8595433
CAACGCA33800.040.7524455
AACGCAG36000.038.4633986
TATCAAC38000.036.248232
GTACATG39600.035.224611
ACGCAGA40850.033.5418137
CGCAGAG41250.033.040818
TACATGG40300.032.920232
CTTACGC900.003629386432.220653
ACATGGG43200.030.3746743
GCAGAGT47200.027.9541429
GAGTACA29250.026.8248921
CATGGGG32450.026.362354
GTGGTAT10900.025.3277851
TTACGCT1450.001226935724.998784
TACCGTG1600.002176020322.6551447
CAGAGTA46500.022.6064229
Produced by FastQC (version 0.11.5)